What Is A Person Called That Draws Blood
This affiliate covers all the steps recommended for safe phlebotomy and reiterates the accustomed principles for blood cartoon and blood drove (31). The chapter includes groundwork data (Section ii.1), applied guidance (Section 2.2) and illustrations (Section 2.3) relevant to best practices in phlebotomy.
The information given in this section underpins that given in the remainder of Part Ii for specific situations. Chapter four also provides data relevant to the procedure for drawing blood given below in Section 2.2, but focuses on claret drove from donors.
Institutions tin use these guidelines to plant standard operating procedures. Such procedures should conspicuously state the risks to patients and wellness workers, too as the means to reduce those risks – discussed below in Sections 2.1.iv and 2.2.
2.1. Background information on best practices in phlebotomy
Best practices in phlebotomy involve the following factors:
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planning ahead;
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using an appropriate location;
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standards for quality care for patients and health workers, including
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availability of appropriate supplies and protective equipment;
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availability of post-exposure prophylaxis (PEP);
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avoidance of contaminated phlebotomy equipment;
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appropriate training in phlebotomy;
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cooperation on the part of patients;
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quality of laboratory sampling.
two.ane.i. Planning ahead
This is the virtually important part of conveying out any procedure, and is usually done at the start of a phlebotomy session.
ii.i.2. Using an appropriate location
The phlebotomist should piece of work in a tranquillity, clean, well-lit area, whether working with outpatients or inpatients.
2.ane.3. Quality control
Quality balls is an essential part of best practice in infection prevention and control (1). In phlebotomy, it helps to minimize the chance of a mishap. Table 2.1 lists the primary components of quality balls, and explains why they are important.
Tabular array 2.i
Elements of quality assurance in phlebotomy.
2.ane.4. Quality intendance for patients and health workers
Several factors tin improve safety standards and quality of care for both patients and health workers, and laboratory tests. These factors, discussed below, include:
Availability of appropriate supplies and protective equipment
Procurement of supplies is the direct responsibility of the administrative (management) structures responsible for setting upward phlebotomy services. Management should:
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provide hand-hygiene materials (lather and water or alcohol rub), well-fitting non-sterile gloves, single-use disposable needles, and syringes or lancing devices in sufficient numbers to ensure that each patient has a sterile needle and syringe or equivalent for each blood sampling;
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brand available sufficient laboratory sample tubes to prevent dangerous practices (e.g. decanting blood to recycle laboratory tubes).
Several safe-engineered devices are available on the market; such devices reduce exposure to blood and injuries. However, the apply of such devices should be accompanied by other infection prevention and control practices, and training in their use. Not all safety devices are applicable to phlebotomy. Before selecting a condom-engineered device, users should thoroughly investigate available devices to determine their appropriate use, compatibility with existing phlebotomy practices, and efficacy in protecting staff and patients (12, 33). Annex B provides further data on infection prevention and control, safe equipment and best do; Annex C provides a comprehensive guide to devices available for drawing blood, including safety-engineered equipment.
For settings with low resources, cost is a driving factor in procurement of safety-engineered devices.
Where prophylactic-engineered devices are not available, skilled use of a needle and syringe is acceptable.
Availability of post-exposure prophylaxis
Adventitious exposure and specific information nearly an incident should be recorded in a register.
Back up services should exist promoted for those who undergo accidental exposure. PEP can assistance to avert HIV and hepatitis B infections (13, 27). Hepatitis B immunization should exist provided to all health workers (including cleaners and waste handlers), either upon entry into wellness-care services or as office of PEP (34). Annex D has details of PEP for hepatitis B and HIV.
Avoidance of contaminated phlebotomy equipment
Tourniquets are a potential source of methicillin-resistant Staphylococcus aureus (MRSA), with up to 25% of tourniquets contaminated through lack of paw hygiene on the function of the phlebotomist or reuse of contaminated tourniquets (35). In add-on, reusable finger-prick devices and related point-of-care testing devices (east.thousand. glucometers) contaminated with blood have been implicated in outbreaks of hepatitis B (4, 5, 36).
To avoid contamination, whatever common-use items, such as glucometers, should be visibly clean earlier use on a patient, and single-apply items should not be reused.
Training in phlebotomy
All staff should be trained in phlebotomy, to forbid unnecessary risk of exposure to blood and to reduce adverse events for patients.
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Groups of health workers who historically are non formally trained in phlebotomy should be encouraged to have up such training; lax infection prevention and control practices outcome in poor prophylactic for staff and take a chance to patients (20, 37).
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The length and depth of training will depend on local conditions; withal, the grooming should at least encompass the essentials (see Addendum Eastward) (38).
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Supervision by experienced staff and structured training is necessary for all wellness workers, including physicians, who undertake blood sampling.
Patient cooperation
1 of the essential markers of quality of care in phlebotomy is the involvement and cooperation of the patient; this is mutually benign to both the health worker and the patient.
Clear information – either written or exact – should be available to each patient who undergoes phlebotomy. Annex F provides sample text for explaining the blood-sampling process to a patient.
2.i.5. Quality of laboratory sampling
Factors that influence the outcome of laboratory results during collection and transportation include:
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knowledge of staff involved in claret collection;
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use of the correct guess of hypodermic needle (see Tabular array iii.one in Chapter iii) to prevent haemolysis or aberrant results;
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the anatomical insertion site for venepuncture;
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the utilise of recommended laboratory drove tubes;
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patient–sample matching (i.due east. labelling);
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transportation conditions;
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interpretation of results for clinical direction.
2.2. Applied guidance on best practices in phlebotomy
2.2.one. Provision of an advisable location
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In an outpatient department or clinic, provide a defended phlebotomy cubicle containing:
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a clean surface with two chairs (one for the phlebotomist and the other for the patient);
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a hand wash basin with lather, running h2o and paper towels;
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alcohol mitt rub.
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In the blood-sampling room for an outpatient department or clinic, provide a comfortable reclining burrow with an arm residual.
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In inpatient areas and wards:
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at the patient's bedside, close the bed curtain to offer privacy
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ensure that blood sampling is done in a private and make clean manner.
2.2.2. Provision of articulate instructions
Ensure that the indications for blood sampling are clearly divers, either in a written protocol or in documented instructions (east.one thousand. in a laboratory form).
2.2.3. Procedure for drawing claret
At all times, follow the strategies for infection prevention and control listed in Table 2.2.
Table 2.2
Infection prevention and control practices.
Stride 1. Get together equipment
Collect all the equipment needed for the procedure and place it within safe and easy reach on a tray or trolley, ensuring that all the items are conspicuously visible. The equipment required includes:
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a supply of laboratory sample tubes, which should be stored dry and upright in a rack; blood can be collected in
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sterile glass or plastic tubes with safe caps (the option of tube will depend on what is agreed with the laboratory);
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vacuum-extraction blood tubes; or
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glass tubes with screw caps;
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a sterile glass or bleeding pack (collapsible) if big quantities of blood are to be collected;
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well-fitting, non-sterile gloves;
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an array of blood-sampling devices (safety-engineered devices or needles and syringes, meet below), of different sizes;
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a tourniquet;
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booze mitt rub;
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gauze or cotton-wool ball to be applied over puncture site;
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laboratory specimen labels;
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writing equipment;
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laboratory forms;
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leak-proof transportation bags and containers;
Ensure that the rack containing the sample tubes is close to you, the health worker, but away from the patient, to avoid it being accidentally tipped over.
Step ii. Identify and prepare the patient
Where the patient is adult and witting, follow the steps outlined beneath.
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Introduce yourself to the patient, and ask the patient to country their full name.
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Check that the laboratory grade matches the patient'due south identity (i.e. match the patient's details with the laboratory class, to ensure accurate identification).
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Ask whether the patent has allergies, phobias or has ever fainted during previous injections or claret draws.
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If the patient is anxious or afraid, reassure the person and ask what would make them more comfortable.
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Make the patient comfortable in a supine position (if possible).
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Place a make clean newspaper or towel under the patient's arm.
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Discuss the test to be performed (see Annex F) and obtain verbal consent. The patient has a right to pass up a test at whatever time before the claret sampling, so it is important to ensure that the patient has understood the procedure.
For paediatric or neonatal patients, meet Chapter 6.
Step 3. Select the site
Full general
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Extend the patient'southward arm and inspect the antecubital fossa or forearm.
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Locate a vein of a good size that is visible, directly and clear. The diagram in Section 2.three, shows common positions of the vessels, but many variations are possible. The median cubital vein lies between muscles and is normally the most like shooting fish in a barrel to puncture. Under the basilic vein runs an avenue and a nervus, so puncturing here runs the risk of dissentious the nervus or avenue and is unremarkably more painful. Practice Not insert the needle where veins are diverting, because this increases the run a risk of a haematoma.
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The vein should be visible without applying the tourniquet. Locating the vein volition help in determining the right size of needle.
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Apply the tourniquet about 4–5 finger widths to a higher place the venepuncture site and re-examine the vein.
Hospitalized patients
In hospitalized patients, do not have blood from an existing peripheral venous access site because this may give false results. Haemolysis, contamination and presence of intravenous fluid and medication can all change the results (39). Nursing staff and physicians may access central venous lines for specimens following protocols. However, specimens from central lines carry a risk of contagion or erroneous laboratory test results.
Information technology is acceptable, only not ideal, to draw blood specimens when first introducing an in-dwelling house venous device, before connecting the cannula to the intravenous fluids.
Step 4. Perform hand hygiene and put on gloves
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wash easily with soap and water, and dry with single-use towels; or
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if hands are not visibly contaminated, clean with booze rub – utilise 3 ml of booze rub on the palm of the manus, and rub information technology into fingertips, dorsum of hands and all over the hands until dry out.
Step 5. Disinfect the entry site
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Unless drawing claret cultures, or prepping for a blood drove, clean the site with a seventy% alcohol swab for 30 seconds and allow to dry completely (30 seconds) (40–42).
Note: alcohol is preferable to povidone iodine, because blood contaminated with povidone iodine may falsely increase levels of potassium, phosphorus or uric acrid in laboratory examination results (six, vii).
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Apply firm but gentle force per unit area. Commencement from the heart of the venepuncture site and piece of work downwards and outwards to cover an area of ii cm or more than.
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Let the expanse to dry. Failure to permit enough contact time increases the run a risk of contamination.
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DO Non touch the cleaned site; in detail, DO Non identify a finger over the vein to guide the shaft of the exposed needle. It the site is touched, echo the disinfection.
Step 6. Take claret
Venepuncture
Perform venepuncture as follows.
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Anchor the vein by belongings the patient'south arm and placing a thumb BELOW the venepuncture site.
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Enquire the patient to form a fist so the veins are more prominent.
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Enter the vein swiftly at a 30 degree angle or less, and continue to innovate the needle along the vein at the easiest angle of entry.
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Once sufficient blood has been collected, release the tourniquet BEFORE withdrawing the needle. Some guidelines suggest removing the tourniquet as soon as claret flow is established, and always earlier it has been in place for two minutes or more than.
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Withdraw the needle gently and apply gentle pressure to the site with a clean gauze or dry out cotton-wool ball. Ask the patient to hold the gauze or cotton fiber wool in place, with the arm extended and raised. Ask the patient NOT to bend the arm, because doing and so causes a haematoma.
Step 7. Fill the laboratory sample tubes
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When obtaining multiple tubes of claret, use evacuated tubes with a needle and tube holder. This system allows the tubes to be filled directly. If this system is not bachelor, use a syringe or winged needle set instead.
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If a syringe or winged needle set is used, best do is to place the tube into a rack before filling the tube. To prevent needle-sticks, use one hand to make full the tube or apply a needle shield between the needle and the mitt holding the tube.
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Pierce the stopper on the tube with the needle directly to a higher place the tube using dull, steady pressure. Exercise not press the syringe plunger because additional force per unit area increases the risk of haemolysis.
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Where possible, keep the tubes in a rack and movement the rack towards you. Inject downwards into the advisable coloured stopper. Exercise Not remove the stopper considering it will release the vacuum.
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If the sample tube does non have a prophylactic stopper, inject extremely slowly into the tube every bit minimizing the pressure level and velocity used to transfer the specimen reduces the take a chance of haemolysis. Exercise NOT epitomize and remove the needle.
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Before acceleration, invert the tubes containing additives for the required number of times (as specified past the local laboratory).
Footstep 8. Draw samples in the right order
Draw blood collection tubes in the correct guild, to avert cross-contamination of additives betwixt tubes. Equally colour coding and tube additives may vary, verify recommendations with local laboratories. For illustration purposes, Tabular array two.3 shows the revised, simplified recommended order of draw for vacuum tubes or syringe and needle, based on United States National Committee Clinical Laboratory Standards consensus in 2003 (43).
Table 2.3
Recommended club of draw for plastic vacuum tubes.
Step 9. Make clean contaminated surfaces and complete patient procedure
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Discard the used needle and syringe or claret sampling device into a puncture-resistant sharps container.
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Check the label and forms for accurateness. The characterization should be conspicuously written with the information required past the laboratory, which is typically the patient's first and last names, file number, date of birth, and the appointment and time when the blood was taken.
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Discard used items into the appropriate category of waste matter. Items used for phlebotomy that would non release a drop of blood if squeezed (east.thousand. gloves) may be discarded in the full general waste, unless local regulations land otherwise.
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Recheck the labels on the tubes and the forms before dispatch.
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Inform the patient when the procedure is over.
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Ask the patient or donor how they are feeling. Cheque the insertion site to verify that it is not haemorrhage, and then thank the patient and say something reassuring and encouraging before the person leaves.
Step 10. Prepare samples for transportation
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Pack laboratory samples safely in a plastic leak-proof bag with an exterior compartment for the laboratory asking form. Placing the requisition on the exterior helps avoid contamination.
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If there are multiple tubes, place them in a rack or padded holder to avoid breakage during transportation.
Step 11. Clean up spills of blood or trunk fluids
If blood spillage has occurred (e.g. because of a laboratory sample breaking in the phlebotomy surface area or during transportation, or excessive bleeding during the procedure), clean information technology upwards. An example of a condom process is given below.
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Put on gloves and a gown or frock if contamination or bleaching of a uniform is likely in a large spill.
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Mop up liquid from large spills using paper towels, and place them into the infectious waste.
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Remove every bit much blood as possible with moisture cloths earlier disinfecting.
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Appraise the surface to encounter whether information technology will be damaged by a bleach and water solution.
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For cement, metal and other surfaces that tin can tolerate a stronger bleach solution, food the area with an approximately 5000 parts per million (ppm) solution of sodium hypochlorite (1:x dilution of a 5.25% chlorine bleach to water). This is the preferred concentration for large spills (44). Leave the area moisture for 10 minutes.
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For surfaces that may be corroded or discoloured by a strong bleach, make clean carefully to remove all visible stains. Make a weaker solution and leave it in contact for a longer period of time. For example, an approximately 525 ppm solution (1:100 dilution of 5.25% bleach) is effective.
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Prepare bleach solution fresh daily and keep it in a closed container because it degrades over time and in contact with the sun.
If a person was exposed to blood through nonintact peel, mucous membranes or a puncture wound, complete an incident study, as described in WHO best practices for injections and related procedures toolkit. For transportation of claret samples outside a hospital, equip the transportation vehicle with a blood spillage kit. Annex H has farther information on dealing with a claret spillage.
ii.3. Illustrations for best practices in phlebotomy
Effigy 2.2 Filling tubes
What Is A Person Called That Draws Blood,
Source: https://www.ncbi.nlm.nih.gov/books/NBK138665/
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